Evaluating the effect of immune cells on the outcome of patients with mesothelioma

Chee SJ, Lopez M, Mellows T3, Gankande S, Moutasim KA, Harris S, Clarke J, Vijayanand P, Thomas GJ1, Ottensmeier CH

Abstract

BACKGROUND:
We systematically assessed the prognostic and predictive value of infiltrating adaptive and innate immune cells in a large cohort of patients with advanced mesothelioma.
METHODS:
A tissue microarray from 302 samples was constructed. Markers of adaptive immune response in T-cells (CD8+, FOXP3+, CD4+, CD45RO+, CD3+) and B-cells (CD20+), and of innate immune response; neutrophils (NP57+), natural killer cells (CD56+) and macrophages (CD68+) were evaluated.
RESULTS:
We found that in the epithelioid tumours, high CD4+ and CD20+ counts, and low FOXP3+, CD68+ and NP57+ counts linked to better outcome. In the non-epithelioid group low CD8+ and low FOXP3+ counts were beneficial.On multivariate analysis low FOXP3+ remained independently associated with survival in both groups. In the epithelioid group additionally high CD4+, high CD20+, and low NP57+ counts were prognostic.
CONCLUSIONS:
Our data demonstrate for the first time, in predominately advanced disease, the association of key markers of adaptive and innate immunity with survival and the differential effect of histology. A better understanding of the immunological drivers of the different subtypes of mesothelioma will assist prognostication and disease-specific clinical decision-making.British Journal of Cancer advance online publication: 17 August 2017; doi:10.1038/bjc.2017.269 www.bjcancer.com.