Light Fluence Rate and Tissue Oxygenation (St O2 ) Distributions Within the Thoracic Cavity of Patients Receiving Intraoperative Photodynamic Therapy for Malignant Pleural Mesothelioma.

Photochemistry and Photobiology 2020 February 12 [Link]

Dupre PJ, Ong YH, Friedberg J, Singhal S, Carter S, Simone CB 2nd, Finlay JC, Zhu TC, Cengel KA, Busch TM

Abstract

The distributions of light and tissue oxygenation (S_t O₂ ) within the chest cavity were determined for 15 subjects undergoing macroscopic complete resection followed by intraoperative photodynamic therapy (PDT) as part of a clinical trial for the treatment of malignant pleural mesothelioma (MPM). Over the course of light delivery, detectors at each of eight different sites recorded exposure to variable fluence rate. Nevertheless, the treatment-averaged fluence rate was similar among sites, ranging from a median of 40 to 61 mW/cm² during periods of light exposure to a detector. S_t O₂ at each tissue site varied by subject, but posterior mediastinum and posterior sulcus were the most consistently well oxygenated (median S_t O₂ >90%; interquartile ranges ~85%-95%). PDT effect on S_t O₂ was characterized as the S_t O₂ ratio (post-PDT S_t O₂ /pre-PDT S_t O₂ ). High S_t O₂ pre-PDT was significantly associated with oxygen depletion (S_t O₂ ratio<1); although, the extent of oxygen depletion was mild (median S_t O₂ ratio of 0.8). Overall, PDT of the thoracic cavity resulted in moderate treatment-averaged fluence rate that was consistent among treated tissue sites, despite instantaneous exposure to high fluence rate. Mild oxygen depletion after PDT was experienced at tissue sites with high pre-PDT S_t O₂ , which may suggest the presence of a treatment effect.